Biotechnology - Proteomics: Principles and Techniques (English)

   Wednesday, April 02, 2025   

Starting a new Lecture Notes Series on Biotechnology - Proteomics: Principles and Techniques

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Biotechnology - Proteomics: Principles and Techniques By Lecture Notes together!

Lecture 1: Mod-01 Lec-01 Introduction to Proteomics Course

Lecture 2: Mod-02 Lec-02 Central Dogma: Basics of DNA, RNA, Proteins

Lecture 3: Mod-03 Lec-03 Genomics and Transcriptomics: Why proteomics?

Lecture 4: Mod-04 Lec-04 Proteins: Amino acids and structural levels of proteins

Lecture 5: Mod-05 Lec-05 Proteins: Folding and misfolding

Lecture 6: Mod-06 Lec-06 Protein Purification and Peptide Isolation using Chromatography

Lecture 7: Mod-07 Lec-07 Enzymes: Basic concepts, Catalytic and Regulatory strategies

Lecture 8: Mod-08 Lec-08 Proteomics and Systems Biology

Lecture 9: Mod-09 Lec-09 Sample preparation for proteomics applications

Lecture 10: Mod-10 Lec-10 Sample preparation for proteomics applications: Serum and bacterial proteome

Lecture 11: Mod-11 Lec-11 Sample preparation for proteomics applications

Lecture 12: Mod-12 Lec-12 Gelbased Proteomics

Lecture 13: Mod-13 Lec-13 Gel-based Proteomics Two-dimensional electrophoresis(continued)

Lecture 14: Mod-14 Lec-14 Gel-based Proteomics Two-dimensional electrophoresis Workflow

Lecture 15: Mod-15 Lec-15 Two-dimensional electrophoresis: Image processing and data analysis

Lecture 16: Mod-16 Lec-16 Two-dimensional difference gel electrophoresis (2D DIGE)

Lecture 17: Mod-17 Lec-17 Difference gel electrophoresis (continued) Discussion and data analysis

Lecture 18: Mod-18 Lec-18 Applications of two dimensional electrophoresis

Lecture 19: Mod-19 Lec-19 Applications of 2-DE and DIGE

Lecture 20: Mod-20 Lec-20 Fundamentals of mass spectrometry

Lecture 21: Mod-21 Lec-21 Matrix assisted laser desorption/ionization-Time of Flight (MALDI-TOF)

Lecture 22: Mod-22 Lec-22 Liquid chromatography-Mass spectrometry (LC-MS/MS)

Lecture 23: Mod-23 Lec-23 Hybrid-MS/MS Configurations

Lecture 24: Mod-24 Lec-24 Quantitative Proteomics: Stable Isotope Labeling by Amino Acids in Cell Culture(SILAC)

Lecture 25: Mod-25 Lec-25 Quantitative Proteomics: iTRAQ and TMT

Lecture 26: Mod-26 Lec-26 Interactomics: Yeast Two-Hybrid Immunoprecipitation Protein microarrays

Lecture 27: Mod-27 Lec-27 Microarray workflow: Label-based detection techniques

Lecture 28: Mod-28 Lec-28 Microarray related concepts: Recombinational cloning Cell-free expression

Lecture 29: Mod-29 Lec-29 Cell-free synthesis based protein microarrays

Lecture 30: Mod-30 Lec-30 Generating Protein Microarrays: Focus on Nucleic Acid Programmable Protein Array

Lecture 31: Mod-31 Lec-31 Microarray work-flow: Image scanning and processing

Lecture 32: Mod-32 Lec-32 Microarray work-flow: Data analysis

Lecture 33: Mod-33 Lec-33 Applications of protein microarrays

Lecture 34: Mod-34 Lec-34 Applications of cell free protein microarrays

Lecture 35: Mod-35 Lec-35 Label-free techniques: SPR and SPRi

Lecture 36: Mod-36 Lec-36 Label-free techniques: SPRi, Ellipsometry, Interference

Lecture 37: Mod-37 Lec-37 Surface Plasmon Resonance: Biacore SPR and data analysis

Lecture 38: Mod-38 Lec-38 Nanotechniques in proteomics

Lecture 39: Mod-39 Lec-39 Detection System: Diffraction-based biosensors

Lecture 40: Mod-40 Lec-40 Proteomics: Advances and Challenges